Reveal and understand complexity of biological systems
RNA gene-expression gives a unique insight into the cellular responses to stimuli: from tissue down to single cell resolution.
It is a fascinating time to study gene-regulation, since the possibilities are nearly endless. Low input amounts or low sample quality used to be detrimental to your study. For those challenging samples, as well as any RNA species imaginable, we provide dedicated workflows that will lead you directly to publication-ready data.
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Benefits of RNA sequencing
We offer more than just expression analyses. We can guide you from the initial set-up of your experiment to extended data-analysis. Our RNA applications are offered under the laboratory accreditation, ISO/IEC 17025 (L518). We guarantee that we deliver high quality results, for R&D and diagnostic samples alike.
- High coverage sequencing
- Detection of novel genes
- Increased diagnostic yield
- Fast turnaround time
- Low input possible
RNA Sequencing Services
Gene expression analysis
Total transcriptome
Ultra-low Input Transcriptomics
FFPE RNAseq
Single Cell Sequencing
Service workflow
1. Initial meeting
When you consider one of our RNA seq services, our scientific support team discusses various options with you for a project design that addresses the needs of your project optimally. You agree on project costs, deliverables, sample specifications, and data analysis options.
2. Library prep / QC
After your sample arrives at our laboratories, we perform an entry QC to ensure the samples are of sufficient quality for sample preparation and provide you with a report. Based on the outcome, we continue with library preparation and apply a second QC step to ensure sufficient library quality for sequencing.
3. Sequencing
Sequencing of RNAseq projects is performed on the Illumina NovaSeq platform. This takes hours up to two days, depending on the instrument. Detailed information of the sequencing instruments at GenomeScan can be found here.
4. Data QC
The raw data that is generated by the sequencer must be divided to obtain one data-file per sample. This is called demultiplexing. Your project manager checks the data quality by analyzing the quality metrics of the run. Final inspection of the data takes place and the dataset is transferred onto a Hard Disk.
5. Project report
You receive a Project Report together with your data. This report contains an overview of the experimental methods used and sample-related specifications. This includes the quantity and quality specifications of your samples after each part of the workflow. The report summarizes all technical information for publication and provides details for the bioinformatician that will analyze your project.
6. Advanced data analysis
Our experienced Bioinformaticians have developed software for common data analysis requests. These data analysis pipelines are tested and optimized to get the most out of your FASTQ files. Use our validated bioinformatics services to save time and development costs!
Let's get the conversation started for your next NGS project
Please either fill in this form of email us directly at info@genomescan.nl
and we will get in touch with you to discuss your requirements